Research Assistant in In-Cell NMR of RNA Dynamics

The Department of Medical Biochemistry and Microbiology (IMBIM) at Uppsala University is a broad international environment for research and teaching. At IMBIM, basic research is conducted in biochemistry, cell and molecular biology, tumor biology, comparative genetics, functional genomics, immunology, bacteriology, and virology. The department's research is primarily fundamental in nature, but has clear connections and significance for health and disease in both animals and humans. For more information, visit Research – Department of Medical Biochemistry and Microbiology – Uppsala University. The position is located in the Petzold group, which studies RNA structure, dynamics, and function using NMR spectroscopy, chemical probing, and computational methods. Project Description The project aims to understand how RNA structure and dynamics contribute to gene regulation in the cellular environment. A particular focus is on developing and applying in-cell NMR to quantify RNA conformational dynamics, including exchange between ground and excited states, directly in living human cells. Using enzymatic circularization of RNA to counteract intracellular degradation, and optimized relaxation-based data acquisition (selective excitation, SOFAST, and R1ρ relaxation dispersion), the project will map how the cellular environment shapes the structural ensemble of biologically relevant RNA molecules. Duties The work is primarily experimental (laboratory and NMR work) and includes: Preparation and purification of isotope-labeled RNA and DNA samples for NMR (in vitro transcription, large-scale production, and enzymatic circularization of RNA hairpins) Introduction of nucleic acids into human cell lines (e.g., electroporation) and preparation of in-cell NMR samples, including controls for cell viability and leakage (supernatant control) Collection and optimization of solution NMR experiments for nucleic acids, including imino proton detection with selective excitation/SOFAST and measurement of longitudinal relaxation (selT1) Measurement of RNA dynamics using 1H and 15N R1ρ relaxation dispersion to quantify populations, exchange rates, and chemical shifts of excited states, in vitro and in-cell Quantification of intracellular nucleic acid concentration and subcellular localization Processing, curve fitting, and analysis of relaxation and dispersion data Documentation and presentation of results Work is conducted in close collaboration with experimental and computationally-oriented researchers in the group. Qualifications The applicant should have a master's degree in structural biology, biophysics, biochemistry, chemistry, molecular biology, or a related field. Documented practical experience with biomolecular NMR and with preparation of RNA and/or DNA samples for structural studies is required. Experience with in-cell NMR of nucleic acids Experience with RNA dynamics, relaxation dispersion (R1ρ), and analysis of excited states Experience with enzymatic RNA production and circularization and isotope labeling Experience with mammalian cell culture and electroporation/transfection Familiarity with NMR pulse sequences and data analysis (e.g., TopSpin, curve fitting) Scientific writing and publications The applicant should be analytical, meticulous, and have good collaboration skills. Excellent knowledge of English, both spoken and written, is required. About the Position Fixed-term employment until August 31, 2027. Full-time position. Start date as soon as possible or by agreement. Work location: Uppsala. Information about the position is provided by: Professor Katja Petzold, [email protected]. Welcome to submit your application by August 28, 2026. UFV-PA 2026/2237 Uppsala University is a broad research university with a strong international standing. The ultimate goal is to conduct education and research of the highest quality and relevance to make a difference in society. Our greatest asset is all 7,500 employees and 53,000 students who, with curiosity and commitment, make Uppsala University one of the country's most exciting workplaces. Read more about our benefits and what it is like to work at Uppsala University https://uu.se/om-uu/jobba-hos-oss/ The position may be subject to security vetting. Security vetting is a requirement for employment; applicants must be approved. We decline offers of recruitment and advertising assistance. Applications are received through Uppsala University's recruitment system. Trade union representatives: Saco-S - [email protected], Seko - [email protected], ST (OFR/S) - [email protected] Open to All We focus on your competence, not your other circumstances. We are open to adapting the role or workplace to suit your needs.

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